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Biblioteca(s): |
Embrapa Pantanal. |
Data corrente: |
11/11/2019 |
Data da última atualização: |
06/01/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
BEZERRA, A. de O.; NICACIO, A. C.; MENEZES, G. R. de O.; GOMES, R. da C.; SILVA, L. O. C. da; ROCHA-FRIGONI, N. A. de S.; MINGOTI, G. Z.; LEÃO, B. C. da S.; SILVA, E. V. da C. e.; NOGUEIRA, E. |
Afiliação: |
ALEXANDRE DE OLIVEIRA BEZERRA, UFMS; ALESSANDRA CORALLO NICACIO, CNPGC; GILBERTO ROMEIRO DE OLIVEIRA MENEZE, CNPGC; RODRIGO DA COSTA GOMES, CNPGC; LUIZ OTAVIO CAMPOS DA SILVA, CNPGC; NATHÁLIA ALVES DE SOUZA ROCHA-FRIGONI, UNESP; GISELE ZOCCAL MINGOTI, UNESP; BEATRIZ CAETANO DA SILVA LEÃO, UNESP; ELIANE VIANNA DA COSTA E SILVA, UFMS; ERIKLIS NOGUEIRA, CPAP. |
Título: |
Comparison between in vitro embryo production using Y-sorted sperm and timed artificial insemination with non-sorted sperm to produce crossbred calves. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Animal Reproduction Science, v. 208, 2019. |
DOI: |
10.1016/j.anireprosci.2019.06.013 |
Idioma: |
Inglês |
Conteúdo: |
Due to the increasing use of in vitro embryo production (IVEP) and the importance of crossbreeding for beef production, pregnancy rates of Nelore recipients were evaluated following Fixed Time Embryo Transfer with fresh or vitrified IVEP embryos produced with Y-sorted sperm of Angus bulls (B. taurus) or Fixed Time Artificial Insemination using non-sorted sperm. For IVEP in Experiment 1, oocytes were obtained using Ovum Pick Up (OPU) (n = 84 embryos) or from ovaries from a slaughterhouse (SLAUGHTER, n = 66 embryos). In Experiment 2, with oocytes obtained by OPU, IVEP embryos were fresh (FRESH, n = 271) or after vitrification/warming (VITRIFIED, n=79) and PR was compared with FTAI (n=239). In Experiment 1, cleavage rates were 63.8% and 39.1% for OPU and SLAUGHTER groups, respectively (P=0.02), and blastocyst rates were 30.5% and 14.7%, respectively (P = 0.09). The PR was similar when considering the source of oocytes (OPU=35.7%; SLAUGHTER=25.8%; P = 0.17). In Experiment 2, there was no difference in PR for FRESH or VITRIFIED embryos (34.3% and 30.4%, respectively, P=0.72), but lesser than FTAI (47.7, P = 0.002). It is concluded that the IVEP with Y-sorted sperm associated with vitrification or embryos produced with oocytes from different sources did not affect PR when there was transfer of crossbred embryos into recipients, and can optimize large-scale application of IVEP technology; however, FTAI pregnancy rates with non-sex sorted sperm were greater. |
Palavras-Chave: |
Crossbreeding. |
Thesagro: |
Criopreservação; Cultura In Vitro; Embrião Animal; Inseminação Artificial. |
Thesaurus NAL: |
Artificial insemination; Cryopreservation; Embryo (animal); In vitro fertilization. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/204519/1/Comparison-between-in-vitro-embryo-production-using-Y-sorted-bezerra-2019-1.pdf
|
Marc: |
LEADER 02598naa a2200349 a 4500 001 2114169 005 2020-01-06 008 2019 bl uuuu u00u1 u #d 024 7 $a10.1016/j.anireprosci.2019.06.013$2DOI 100 1 $aBEZERRA, A. de O. 245 $aComparison between in vitro embryo production using Y-sorted sperm and timed artificial insemination with non-sorted sperm to produce crossbred calves.$h[electronic resource] 260 $c2019 520 $aDue to the increasing use of in vitro embryo production (IVEP) and the importance of crossbreeding for beef production, pregnancy rates of Nelore recipients were evaluated following Fixed Time Embryo Transfer with fresh or vitrified IVEP embryos produced with Y-sorted sperm of Angus bulls (B. taurus) or Fixed Time Artificial Insemination using non-sorted sperm. For IVEP in Experiment 1, oocytes were obtained using Ovum Pick Up (OPU) (n = 84 embryos) or from ovaries from a slaughterhouse (SLAUGHTER, n = 66 embryos). In Experiment 2, with oocytes obtained by OPU, IVEP embryos were fresh (FRESH, n = 271) or after vitrification/warming (VITRIFIED, n=79) and PR was compared with FTAI (n=239). In Experiment 1, cleavage rates were 63.8% and 39.1% for OPU and SLAUGHTER groups, respectively (P=0.02), and blastocyst rates were 30.5% and 14.7%, respectively (P = 0.09). The PR was similar when considering the source of oocytes (OPU=35.7%; SLAUGHTER=25.8%; P = 0.17). In Experiment 2, there was no difference in PR for FRESH or VITRIFIED embryos (34.3% and 30.4%, respectively, P=0.72), but lesser than FTAI (47.7, P = 0.002). It is concluded that the IVEP with Y-sorted sperm associated with vitrification or embryos produced with oocytes from different sources did not affect PR when there was transfer of crossbred embryos into recipients, and can optimize large-scale application of IVEP technology; however, FTAI pregnancy rates with non-sex sorted sperm were greater. 650 $aArtificial insemination 650 $aCryopreservation 650 $aEmbryo (animal) 650 $aIn vitro fertilization 650 $aCriopreservação 650 $aCultura In Vitro 650 $aEmbrião Animal 650 $aInseminação Artificial 653 $aCrossbreeding 700 1 $aNICACIO, A. C. 700 1 $aMENEZES, G. R. de O. 700 1 $aGOMES, R. da C. 700 1 $aSILVA, L. O. C. da 700 1 $aROCHA-FRIGONI, N. A. de S. 700 1 $aMINGOTI, G. Z. 700 1 $aLEÃO, B. C. da S. 700 1 $aSILVA, E. V. da C. e. 700 1 $aNOGUEIRA, E. 773 $tAnimal Reproduction Science$gv. 208, 2019.
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Embrapa Pantanal (CPAP) |
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